Customized Photobioreactor ZBP150

Aeration:

• Aeration system provides mixing and also air/CO₂supply to cultures.
• Integrated stainless steel U-tube with aeration holes (Ø 0.7 mm) + silicon connection.
• The air source: Aeration Pump supplemented with a valve, which interrupts bubbling before the OD and fluorescence measurements to avoid any bubble interference with the measurements and thus to allow for more precise readings.
• Optionally, the Photobioreactor ZBP 150 can be linked to the Gas Mixing System GMS 150that provides precise concentration and flow rate of the required gases.

Mixing:

• Mixing either by aeration (see Aeration System for more information) or by a Magnetic Stirrer (optional).
• Two versions of the Magnetic Stirrer are offered (V-200 for FMT 150/400, FMT 150/1000 and V-300 for FMT 150/3000).
• Magnetic stirrerwith 35 mm long (Ø 6 mm) teflon-coated magnetic bar is intended for FMT 150/400 and FMT 150/1000 whereas a 60 mm long (Ø 10 mm) magnetic bar is used for FMT 150/3000). The magnetic bar is positioned in a vertical plane of the front glass panel inside the Photobioreactor. Rotation speed is adjustable approximately from 10 to 100 % (maximal rotation is 600 rpm for FMT 150/400, FMT 150/1000 and 400 rpm for FMT 150/3000).
• The use of magnetic stirrer is essential when the agitation by gas cannot be applied, e.g. when the photosynthetic oxygen evolution or respiratory oxygen uptake are measured or when growing the culture anaerobically without gas supply. It can be also used to reduce cell sedimentation and clumping in addition to agitation by gas bubbling. It is highly recommended with use of the O₂and CO₂ probes based on Clark-type electrodes.

NOTE: The magnetic stirrer can’t be used simultaneously with the External Light Panel and with the PWM pump.

Sensors:

• pH / Temperature Moduleincludes the electronic module, combined membrane sensor and cable. Analogue pH sensor InPro 3253SG/120/PT1000 produced by Mettler Toledo.
• O₂Module (optional) to measure concentration of dissolved O₂ (includes the electronic module, membrane sensor and cable). Analogue O₂ sensor InPro 6800/12/120 produced by Mettler Toledo.
• CO₂sensor (optional) Digital CO₂ sensor InPro 5000i/12/120 produced by Mettler Toledo. To determine the dissolved carbon dioxide concentration in cultivated suspension (includes the electronic module, sensor probe with digital communication, and cables).

Cultivation strategies

• The highly flexible use of the PBS’s system is enabled through connection and control of several additional pumps. Such design allows to use any of the measured parameters (OD, pH, dCO₂or dO₂, etc.) as a control regulator in order to operate the system in automatic regime.
• Up to 8 Peristaltic Pumps PP 600(optional) and two PWM diaphragm pumps (optional) can be linked to the Photobioreactor ZBP 150 for supply of different nutrients, medium, catalysts, inhibitors, toxicants, base or acid, organisms, etc.
• Batch cultivation
• Semi-continuous cultivation
• Turbidostatic cultivation
• Chemostatic cultivation
• Anaerobic cultivation
• Turbidostat Module(optional): maintains a pre-set cell concentration (determined through OD 680 or OD 720) in a constant biomass volume. The pre-set cell concentration can be constant, fluctuating in a various range or dynamically changed. The module includes one Peristaltic Pump PP 600 and supporting control software.
• Chemostat Module(optional): In chemostat mode, pH value of the cultivation media is continuously adjusted by acid or base addition to the cultivation vessel in order to keep pH level on defined level. The module includes two Peristaltic Pumps PP 600 and supporting control software. One pump serves adding acid, the second pump adds base.
• pH-Stat / Turbidostat Module(optional): The Module enables either turbidostatic cultivation or pH-stat mode cultivation regulated through a controlled gaseous CO₂ The Module includes one Peristaltic Pump PP 600 with one gas valve and supporting control software. CO₂ container is not included.
• pH-Stat cultivation through Gas Mixing System GMS 150(optional): The Photobioreactor Control Software for GMS 150 (advanced, optional) enables to create a Java script that will keep the pH of the cultivation medium at defined level through a regulated gaseous CO₂
• Anaerobic cultivationvia gas tight lid and N₂
• Peristaltic Pump 600is used for accurate and homogenous pumping of variety of fluids. The fluid delivery occurs with minimum sheer so the potential damage to the biological material is marginal. Pump flow-rate runs between 0.2 and 50 ml/min. The speed of rotation and thus of pumping can be controlled manually via the pump display, or by a PC via USB interface.
• PWM Pump(for Gas or Liquid depending on the used membrane): PWM pump can be used for pumping liquid or gas into the cultivation cuvette; it can be regulated within a range of 1-100 % of total output. The pump has diverse uses: (1) Controlled air bubbling in the cultivation vessel; (2) Single gas input into the vessel; (3) When connected to the vessel inlet and outlet, it can be used for suspension mixing (bubbling) in anaerobic conditions. NOTE: The PWM pump can’t be used simultaneously with the Magnetic Stirrer.

Photobioreactor controlling software

• The entire Photobioreactor ZBP 150 system and all its cultivation and monitoring functions and accessories can be controlled via the Photobioreactor Control Software. The control software provides intuitive and dynamic interface for online monitoring and visualization of all measured data as well as creation of user-defined protocols. For more details see our website about the Photobioreactor Control Software.

Application

• Highly precise cultivation of microorganisms
• Optimization of diverse cultivation conditions as nutrients, illumination, etc.
• Biomass and end-product yield optimization
• Growth dynamics monitoring
• Regulatory profiling of algae
• Photosynthesis research
• Photosynthetic pigments studies
• Stress physiology, detection of biotic and abiotic stress
• Study of diurnal or metabolic rhythms
• Study of secondary metabolites production
• Bioprospecting of natural populations
• Ecotoxicological research
• Ecological research, microorganisms interactions, population dynamic
• Environmental modeling